Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 920 | ml | - | - | |
Solution B | 50 | ml | - | - | |
Solution C | 30 | ml | - | - | |
1 Dissolve ingredients of solution A and sparge with 80% N2 and 20% CO2 gas atmosphere to make it anoxic. Then distribute medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B is prepared under 100% N2 gas atmosphere and autoclaved. Solution C is prepared under 80% N2 and 20% CO2 gas atmosphere and autoclaved. To complete the medium appropriate amounts of solutions B and C are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 7.4. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
CaCl2 x 2 H2O | 0.1 | g | 0.109 | 0.739 |
MgSO4 x 7 H2O | 0.1 | g | 0.109 | 0.441 |
KH2PO4 | 0.5 | g | 0.543 | 3.994 |
K2HPO4 | 0.5 | g | 0.543 | 3.12 |
NaCl | 1.0 | g | 1.087 | 18.6 |
Clarified rumen fluid | 300.0 | ml | - | - |
Yeast extract
(OXOID) |
0.5 | g | 0.543 | - |
Peptone
(BD BACTO) |
0.5 | g | 0.543 | - |
(NH4)2SO4 | 0.5 | g | 0.543 | 4.113 |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.4e-4 | 0.002 |
L-Cysteine HCl x H2O | 0.5 | g | 0.543 | 3.094 |
Distilled water | 620.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Glucose | 10 | g | 200 | 1110.12 |
Distilled water | 50 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2CO3 | 1.5 | g | 50 | 471.752 |
Distilled water | 30.0 | ml | - | - |
1 Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C. |
Last modified: | 08.09.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.4 |
Equipment needed: | Autoclave, Centrifuge, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Glucose | 10 | 55.506 |
Na2CO3 | 1.5 | 14.153 |
NaCl | 1 | 17.112 |
(NH4)2SO4 | 0.5 | 3.784 |
KH2PO4 | 0.5 | 3.674 |
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