DSMZ 283 . PYRODICTIUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Serum bottle Water bath Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 13.85 g 13.512 231.215
MgSO4 x 7 H2O 3.50 g 3.415 13.854
MgCl2 x 6 H2O 2.75 g 2.683 13.197
KCl 0.33 g 0.322 4.319
NaBr 0.05 g 0.049 0.474
H3BO3 15.00 mg 0.015 0.237
SrCl2 x 6 H2O
(0.1% w/v)
7.00 ml 0.007 0.026
(NH4)2SO4 10.00 mg 0.01 0.074
Citric acid
(0.1% w/v)
5.00 ml 0.005 0.025
KI
(0.01% w/v)
0.50 ml 4.9e-5 1.9e-4
CaCl2 x 2 H2O 0.75 g 0.732 4.977
KH2PO4 0.50 g 0.488 3.585
NiCl2 x 6 H2O
(0.1% w/v)
2.00 ml 0.002 0.015
Modified Wolin's mineral solution 10.00 ml - -
Sulfur
(powdered)
30.00 g 29.268 912.638
Sodium resazurin
(0.1% w/v)
0.50 ml 4.9e-4 0.002
Yeast extract
(OXOID)
2.00 g 1.951 -
Na2S x 9 H2O 0.50 g 0.488 2.031
Distilled water 1000.00 ml - -
1 Prepare the medium without sulfur, yeast extract and sodium sulfide, adjust pH to 5.0 - 5.5 with 10 N sulfuric acid, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under 80% H2 and CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials that contain already the appropriate amount of sulfur, only to 30% of their volume to allow for a large headspace. For sterilization of medium heat vessels for at least 1 hour to 90 - 100°C on each of 3 successive days. Do not autoclave! Complete the medium by adding yeast extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 5.5, if necessary.
2 After inoculation pressurize the vessels to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 27.10.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Gassing station, Serum bottle, Water bath
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 29.268 912.638
MgCl2 x 6 H2O 2.683 13.197
Yeast extract 1.951 -
KH2PO4 0.488 3.585
Na2S x 9 H2O 0.488 2.031
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
temperature hyperthermophilic 3 strains