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298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER)

Culture medium recipe

Vol.
×
Solution occurs in 1 other media (first defined in medium 298 )
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 0.2 1.467
2 0.25 g 0.25 4.664
3 1.00 g 0.998 17.077
4 0.40 g 0.399 1.964
5 0.50 g 0.499 6.693
6 0.15 g 0.15 1.018
7 Trace element solution SL-10 1.00 ml - -
8
(0.1% w/v)
0.50 ml 5.0e-4 0.002
9 1.50 g 1.497 14.124
10 0.90 g 0.898 9.967
11 0.36 g 0.359 1.496
12 1000.00 ml 996.215 55298.2
13 , may stimulate growth of some strains
(5% w/v)
20.00 mg/l 0.02 -
14
(molecular weight 106 - 20000)
optional
1.00 g/l 1 -
15 , might need strain adjustment 1.00 g/l - -
16 , might need strain adjustment 2.60 g/l - -
17 Clarified rumen fluid , might need strain adjustment 20.00 ml/l - -
18 Vitamin solution , might need strain adjustment 10.00 ml/l - -
19 , might need strain adjustment 1.00 g/l - -
21 , might need strain adjustment 2.00 g/l - -
22 Seven vitamins solution , might need strain adjustment 1.00 ml - -
23 , might need strain adjustment
(1 M)
1.40 g/l - -
24 , might need strain adjustment 1.24 g/l - -
25 Selenite-tungstate solution , might need strain adjustment 1.00 ml - -
26 , might need strain adjustment 2.20 g/l - -
27
optional
0.86 g/l 0.86 9.99
28 , might need strain adjustment 1.25 g/l - -
Instructions:
  1. Dissolve ingredients except carbonate, 2,3-butanediol and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.2 - 7.5.
  2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning.
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
Solution occurs in 32 other media (first defined in medium 1310 )
Equipment needed: Centrifuge Filter Autoclave
Instructions:
  1. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 minand then centrifuged at 27,000 g for 20 min The supernatant is made anoxic by sparging with 100% N2 gas for 15 min dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at - 20°C.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Autoclave, Centrifuge, Filter, Hungate tubes

Associated strains

All strains for this medium: 12
Strains with modifications: 12

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.201 55352.9
Na2CO3 1.497 14.124
Polyethylene glycol
optional
1 -
NaCl 0.998 17.077
2,3-butanediol 0.898 9.967
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Cultivation metadata from 12 strains BacDive

phyla 3 12 strains
oxygen anaerobe 12 strains
temperature mesophilic 12 strains

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