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298b. SYNTROPHOBOTULUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 0.198 1.452
2 0.25 g 0.247 4.618
3 1.00 g 0.988 16.908
4 0.40 g 0.395 1.944
5 0.50 g 0.494 6.627
6 0.15 g 0.148 1.008
7 Trace element solution SL-10 1.00 ml - -
8 0.20 g 0.198 2.409
9
(0.1% w/v)
0.50 ml 4.9e-4 0.002
10 1.50 g 1.482 13.985
11 0.60 g 0.593 6.175
12 Vitamin solution 10.00 ml - -
13 0.30 g 0.296 1.688
14 0.30 g 0.296 1.234
15 1000.00 ml 986.371 54751.8
16 , might need strain adjustment 1.00 g/l - -
Instructions:
  1. Dissolve ingredients except carbonate, glyoxalate, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add glyoxalate (sterilized by filtration), vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.2 - 7.5.
  2. Note: When growth has started, feed culture once or twice with the same amount of glyoxalate.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.211 55353.5
Na2CO3 1.482 13.985
NaCl 0.988 16.908
Na2-glyoxalate 0.593 6.175
KCl 0.494 6.627
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains

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