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DSMZ

DSMZ 2 . BACILLUS PASTEURII MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Sporosarcina ureae DSM 2306

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 2 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 1 1000 ml - -
Urea 20 g/l 20 333.023
1 To medium 1 add 20 g/l urea before autoclaving. Do not adjust pH; pH raises to about 8 due to heat degraded urea.
2 For sporulation enhancement add 10 mg/l MnSO4 x H2O.

Medium 1 #

1000
Solution occurs in 20 other media (first defined in Medium 1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Agar , for solid medium 15 g 15 -
Distilled water 1000 ml - -
1 Adjust pH to 7.0.
2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Metadata

Last modified: 04.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 10
Sporosarcina ureae DSM 2306

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Urea 20 333.023
Agar 15 44.599
Peptone 5 -
Meat extract 3 -
Distilled water - -
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Cultivation metadata from 10 strains BacDive

phyla 1 9 strains
temperature mesophilic 9 strains