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3. AZOTOBACTER MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 4.762 26.432
2 5.00 g 4.762 26.14
3 0.10 g 0.095 0.648
4 0.10 g 0.095 0.386
5 5.00 mg 0.005 0.02
6 0.90 g 0.857 4.921
7 0.10 g 0.095 0.7
8 0.01 g 0.01 0.034
9 5.00 g 4.762 47.578
10 15.00 g 14.286 -
11 950.00 ml 903.14 50131.8
Instructions:
  1. Adjust pH to 7.3.
  2. Sterilize glucose and mannitol separately (in 50 ml H2O) and add to the medium after autoclaving. Calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3

Associated strains

All strains for this medium: 41
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 903.14 50131.8
Agar 14.286 -
CaCO3 4.762 47.578
Glucose 4.762 26.432
Mannitol 4.762 26.14
K2HPO4 0.857 4.921
KH2PO4 0.095 0.7
CaCl2 x 2 H2O 0.095 0.648
MgSO4 x 7 H2O 0.095 0.386
FeSO4 x 7 H2O 0.01 0.034
Na2MoO4 x 2 H2O 0.005 0.02

Cultivation metadata from 41 strains BacDive

phyla 1 41 strains
temperature mesophilic 41 strains

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