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DSMZ

DSMZ 316 . MOORELLA THERMOACETICA (TYE-CO) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Moorella thermoacetica DSM 2955

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 55 °C

Main sol. 316 #

ml
Equipment needed: Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.984 18.401
KH2PO4 0.3 g 0.295 2.17
Na2HPO4 x 12 H2O 2.8 g 2.756 19.413
MgCl2 x 6 H2O 0.2 g 0.197 0.968
Trypticase peptone
(BD BBL) 		10.0 g 9.843 -
Yeast extract
(OXOID) 		3.0 g 2.953 -
Modified Wolin's mineral solution 10.0 ml - -
Wolin's vitamin solution 5.0 ml - -
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) 		1.0 ml 9.8e-4 0.004
Sodium resazurin
(0.1% w/v) 		0.5 ml 4.9e-4 0.002
Na2S x 9 H2O 0.6 g 0.591 2.459
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% carbon monoxide gas. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 25.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % CO

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Moorella thermoacetica DSM 2955

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase peptone 9.843 -
Yeast extract 2.953 -
Na2HPO4 x 12 H2O 2.756 19.413
NH4Cl 0.984 18.401
Na2S x 9 H2O 0.591 2.459
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain