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DSMZ 318b . ANAEROTIGNUM NEOPROPIONICUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.30 g 0.3 2.205
NaCl 0.60 g 0.6 10.267
MgCl2 x 6 H2O 0.10 g 0.1 0.492
CaCl2 x 2 H2O 0.08 g 0.08 0.544
Trace element solution 10.00 ml - -
NH4Cl 1.00 g 1 18.695
Yeast extract
(OXOID)
0.50 g 0.5 -
Trypticase peptone
(BD BBL)
0.50 g 0.5 -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
KHCO3 4.00 g 4 39.954
Ethanol 1.30 ml - -
Wolin's vitamin solution 10.00 ml - -
L-Cysteine HCl x H2O 0.30 g 0.3 1.708
Na2S x 9 H2O 0.30 g 0.3 1.249
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, ethanol, vitamins, cysteine and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 minto make it anoxic. Add and dissolve bicarbonate and adjust pH to 7.0, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ethanol, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.0 - 7.2, if necessary.
Solution occurs in 14 other media (first defined in Medium 318).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
12.80 g 12.8 66.967
FeCl2 x 4 H2O 1.00 g 1 5.03
MnCl2 x 4 H2O 0.10 g 0.1 0.505
CoCl2 x 6 H2O 0.03 g 0.03 0.231
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.02 g 0.02 0.149
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
NiCl2 x 6 H2O 0.10 g 0.1 0.772
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.03 g 0.03 0.114
Na2WO4 x 2 H2O 0.04 g 0.04 0.121
Distilled water 1000.00 ml - -
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.
Solution occurs in 351 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes

Gas composition

  • 20 % CO2
  • 80 % N2

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KHCO3 4 39.954
NH4Cl 1 18.695
Yeast extract 0.5 -
Trypticase peptone 0.5 -
KH2PO4 0.3 2.205
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
nutrition chemoorganotroph 1 strain
oxygen anaerobe 1 strain
oxygen obligate anaerobe 1 strain
temperature mesophilic 1 strain

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