DSMZ 31 . ALKALINE NUTRIENT AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Selected strain: Halalkalibacter krulwichiae DSM 18225

This strain uses the standard medium without modifications.

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Agar , if necessary 15 g 15 44.599
Distilled water 900 ml - -
1 After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7
Na-sesquicarbonate solution 100 ml - -
2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
Solution occurs in 1 other media (first defined in Medium 31).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 4.2 g 42 499.958
Na2CO3 anhydrous 5.3 g 53 500.057
Distilled water 100.0 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.7
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 154
Strains with modifications: 68
Halalkalibacter krulwichiae DSM 18225

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2CO3 anhydrous 5.3 50.006
Peptone 5 -
NaHCO3 4.2 49.996
Meat extract 3 -
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Cultivation metadata from 154 strains BacDive

pH 8 - 10 8 strains
pH 6 - 8 5 strains
pH 12 - 14 1 strain
pH 10 - 12 1 strain
pH 4 - 6 1 strain
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