31 .
ALKALINE NUTRIENT AGAR
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* Medium for Bacillus sp. DSM 9820
Plus 0.5% NaCl
Growth conditions: 30 °C
Equipment needed:
Autoclave
Final pH:
9.7
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 5 | g | ||
|
|
Meat extract | 3 | g | ||
|
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Agar , if necessary | 15 | g | ||
|
|
Distilled water | 900 | ml | ||
| 1 After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7 | |||||
|
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Na-sesquicarbonate solution | 100 | ml | ||
|
|
NaCl | 5 | g/l | ||
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Na-sesquicarbonate solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaHCO3 | 4.2 | g |
|
|
Na2CO3 anhydrous | 5.3 | g |
|
|
Distilled water | 100.0 | ml |