31 .
ALKALINE NUTRIENT AGAR
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Selected strain: Bacillus sp. DSM 2519
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Autoclave
Final pH:
9.7
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Peptone | 5 | g | ||
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Meat extract | 3 | g | ||
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Agar , if necessary | 15 | g | ||
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Distilled water | 900 | ml | ||
| 1 After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7 | |||||
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Na-sesquicarbonate solution | 100 | ml | ||
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Na-sesquicarbonate solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaHCO3 | 4.2 | g |
|
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Na2CO3 anhydrous | 5.3 | g |
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Distilled water | 100.0 | ml |