DSMZ 326 . THERMOCLOSTRIDIUM (A-GÖ) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Selected strain: Thermoclostridium stercorarium subsp. thermolacticum DSM 2910

This strain uses the standard medium without modifications.

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 3.30 g 3.293 24.201
MgCl2 x 6 H2O 0.16 g 0.16 0.785
CoCl2 x 6 H2O 12.00 mg 0.012 0.092
Trace element solution SL-10 1.00 ml - -
Yeast extract 2.00 g 1.996 -
Trypticase peptone
(BD BBL)
2.00 g 1.996 -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 0.002
Na2CO3 1.00 g 0.998 9.416
Sucrose 5.00 g 4.99 14.578
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.30 g 0.299 1.705
Na2S x 9 H2O 0.30 g 0.299 1.247
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, sucrose, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add sucrose, cysteine and sulfide to the medium from sterile stock solutions autoclaved under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to pH 6.8 - 7.2, if necessary.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 299 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 25.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
Edit in Medium builder

Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Thermoclostridium stercorarium subsp. thermolacticum DSM 2910

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sucrose 4.99 14.578
KH2PO4 3.293 24.201
Yeast extract 1.996 -
Trypticase peptone 1.996 -
Na2CO3 0.998 9.416
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
temperature thermophilic 3 strains