331
331 .
ACETOBACTEROIDES GLYCINOPHILUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 331 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.13 | g | 0.13 | 0.884 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.984 | |
| Trace element solution | 10.00 | ml | - | - | |
| Wolin's vitamin solution | 5.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Yeast extract | 0.50 | g | 0.5 | - | |
| Glycine | 1.50 | g | 1.5 | 19.981 | |
| KH2PO4 | 3.00 | g | 3 | 22.045 | |
| Na2HPO4 | 5.80 | g | 5.8 | 40.857 | |
| NaHCO3 | 0.50 | g | 0.5 | 5.952 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.2 - 7.4 with NaOH. | |||||
| 2 Prepare the medium anaerobically under 100% nitrogen. Prepare concentrated solutions each of yeast extract, glycine, phosphates, NaHCO3 and Na2S separately and sterilize under nitrogen by autoclaving. | |||||
Solution occurs in 8
other media (first defined in Medium 144).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
12.80 | g | 12.8 | 66.967 | |
| FeCl2 x 4 H2O | 0.20 | g | 0.2 | 1.006 | |
| MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
| CoCl2 x 6 H2O | 0.17 | g | 0.17 | 1.309 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
| CuCl2 | 0.02 | g | 0.02 | 0.149 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| Na2SeO3 x 5 H2O | 0.03 | g | 0.03 | 0.114 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml. | |||||
Solution occurs in 53
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| (DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 - 7.4 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2HPO4 | 5.8 | 40.857 |
| KH2PO4 | 3 | 22.045 |
| Glycine | 1.5 | 19.981 |
| NH4Cl | 1 | 18.695 |
| NaHCO3 | 0.5 | 5.952 |
| Show more | ||
