DSMZ 332 . METHANOCULLEUS BOURGENSIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 1 18.695
K2HPO4 x 3 H2O 0.4 g 0.4 2.297
MgCl2 x 6 H2O 0.1 g 0.1 0.492
Na-formate 5.0 g 5 73.522
Na-acetate 1.0 g 1 12.19
Trypticase peptone
(BD BBL)
1.0 g 1 -
Yeast extract
(OXOID)
1.0 g 1 -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 0.002
L-Cysteine HCl x H2O 0.5 g 0.5 2.847
Na2CO3 1.5 g 1.5 14.153
Na2S x 9 H2O 0.2 g 0.2 0.833
Distilled water 1000.0 ml - -
1 Dissolve ingredients except cysteine, carbonate and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve cysteine, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture and sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary.
2 Note: After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure.

Metadata

Last modified: 07.11.22
Source: DSMZ
Synonyms: METHANOCULLEUS BOURGENSE MEDIUM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.8 - 7.0
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-formate 5 73.522
Na2CO3 1.5 14.153
NH4Cl 1 18.695
Na-acetate 1 12.19
Yeast extract 1 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain