Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
|Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
|K2HPO4 x 3 H2O||0.4||g||0.4||2.297|
|MgCl2 x 6 H2O||0.1||g||0.1||0.492|
|L-Cysteine HCl x H2O||0.5||g||0.5||2.847|
|Na2S x 9 H2O||0.2||g||0.2||0.833|
|1 Dissolve ingredients except cysteine, carbonate and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve cysteine, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture and sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary.|
|2 Note: After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure.|
|Synonyms:||METHANOCULLEUS BOURGENSE MEDIUM|
|Medium type:||Complex medium|
|Final pH:||6.8 - 7.0|
|Equipment needed:||Autoclave, Gassing station, Hungate tubes|
|Edit in Medium builder|
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
|All strains for this medium:||1|
|Compound||Conc. [g/L]||Conc. [mM]|