338 .
CAMPYLOBACTER RECTUS MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
7.5
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Beef extract
(Difco) |
3.00 | g | ||
|
|
Trypticase
(BBL) |
9.00 | g | ||
|
|
Yeast extract | 11.00 | g | ||
|
|
NaCl | 2.00 | g | ||
|
|
Na2HPO4 | 0.40 | g | ||
|
|
Na2CO3 | 0.25 | g | ||
|
|
Haemin | 5.00 | mg | ||
|
|
Na-formate | 2.00 | g | ||
|
|
Na-fumarate | 3.00 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.5. Prepare the medium anaerobically under 100% N2. | |||||
| 2 Autoclave solutions of haemin (dissolved in 0.1 ml 1N NaOH, then diluted with water to 10 ml and neutralized), formate, and fumarate separately under nitrogen. | |||||