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339. WILKINS-CHALGREN ANAEROBE BROTH

Culture medium recipe

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* Modified for Acetanaerobacterium sp. DSM 102114
beta

Strictly anaerobic

Solution occurs in 1 other media (first defined in medium 339 )
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(Oxoid CM0643)
33.0 g/l 33 -
2 1000.0 ml 998.207 55408.8
3
(0.1% w/v)
0.5 ml/l 5.0e-4 0.002
4 0.3 g/l 0.3 1.708
5 , see note
(5% w/v)
20.0 mg/l 0.02 -
7 , might need strain adjustment 2.0 g/l - -
Instructions:
  1. Dissolve 33.0 g/l dehydrated Wilkins-Chalgren medium (Oxoid CM0643) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Add L-cysteine- HCL x H2O (0.3 g/l), dispense the medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving check pH and adjust to pH 6.8 - 7.0, if necessary.
  2. Note: In case resazurin is not colourless addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) can be used to completely reduce the medium.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 143
Strains with modifications: 131
Acetanaerobacterium sp. DSM 102114

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Dehydrated Wilkins-Chalgren medium 33 -
L-Cysteine HCl x H2O 0.3 1.708
Sodium dithionite 0.02 -
Na-resazurin 5.0e-4 0.002

Cultivation metadata from 143 strains BacDive

halophily not 2 strains
halophily slightly 1 strain
pH 6 - 8 8 strains
pH 8 - 10 7 strains
pH 4 - 6 6 strains
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Similar media

301. FALCIVIBRIO MEDIUM 94 %
339a. WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) 87 %

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