Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
This strain uses the standard medium without modifications.
|Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
dehydrated Wilkins-Chalgren medium
|1 Dissolve 33.0 g/l dehydrated Wilkins-Chalgren medium (Oxoid CM0643) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving check pH and adjust to pH 6.8 - 7.0, if necessary.|
|2 Note: In case resazurin is not colourless addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) can be used to completely reduce the medium.|
|Medium type:||Complex medium|
|Final pH:||6.8 - 7.0|
|Equipment needed:||Autoclave, Filter, Gassing station, Hungate tubes|
|Edit in Medium builder|
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
|All strains for this medium:||168|
|Strains with modifications:||61|
No modifications necessary for this strain.
|Compound||Conc. [g/L]||Conc. [mM]|
|dehydrated Wilkins-Chalgren medium||33||-|