DSMZ 339a . WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Clostridium bowmanii DSM 14206

This strain uses the standard medium without modifications.

Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
dehydrated Wilkins-Chalgren medium
(Oxoid CM0643)
33.0 g 33 -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
L-Cysteine HCl 0.3 g 0.3 1.903
D-Glucose 5.0 g 5 27.753
Na2CO3 1.0 g - -
Distilled water 1000.0 ml - -
1 Dissolve 33.0 g/l dehydrated Wilkins-Chalgren medium (Oxoid CM0643) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving supplement medium with 5.0 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.8 with a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere.
2 Note: In case resazurin is not colourless addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) can be used to completely reduce the medium.

Metadata

Last modified: 21.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 35
Strains with modifications: 8
Clostridium bowmanii DSM 14206

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
dehydrated Wilkins-Chalgren medium 33 -
D-Glucose 5 27.753
L-Cysteine HCl 0.3 1.903
Sodium resazurin 5.0e-4 -
Distilled water - -

Cultivation metadata from 35 strains BacDive

halophily non-halophilic 1 strain
pH 6 - 8 5 strains
pH 8 - 10 3 strains
pH 4 - 6 2 strains
phyla 4 32 strains
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