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DSMZ

DSMZ 343a . THERMOSIPHO MELANESIENSIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Thermosipho melanesiensis DSM 12029

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 70 °C

Main sol. 343a #

ml
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Starch
(soluble) 		5.0 g 5 -
KH2PO4 0.5 g 0.5 3.674
Modified Wolin's mineral solution 15.0 ml - -
NiCl2 x 6 H2O
(0.1% w/v) 		2.0 ml 0.002 0.015
NaCl 20.0 g 20 342.231
Artificial sea water 250.0 ml - -
Yeast extract
(OXOID) 		2.0 g 2 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 -
L-Cysteine HCl x H2O 0.5 g 0.5 2.847
Na2S x 9 H2O 0.5 g 0.5 2.082
Distilled water 750.0 ml - -
1 Dissolve ingredients (except sulfide and cysteine) and adjust pH to 6.5. Boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of volume and autoclave. Add sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 6.5, if necessary.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Artificial sea water #

1000
Solution occurs in 4 other media (first defined in Medium 343).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.70 g 27.7 473.99
MgSO4 x 7 H2O 7.00 g 7 28.4
MgCl2 x 6 H2O 5.50 g 5.5 27.053
KCl 0.65 g 0.65 8.719
NaBr 0.10 g 0.1 0.972
H3BO3 30.00 mg 0.03 0.485
SrCl2 x 6 H2O 15.00 mg 0.015 0.056
Citric acid 10.00 mg 0.01 0.052
KI 0.05 mg 5.0e-5 1.9e-4
CaCl2 x 2 H2O 2.25 g 2.25 15.305
Distilled water 1000.00 ml - -

Metadata

Last modified: 06.09.22
Source: DSMZ
Synonyms: Thermosipho melanesiensis MEDIUM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Thermosipho melanesiensis DSM 12029

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 6.925 118.497
Starch 5 -
Yeast extract 2 -
MgSO4 x 7 H2O 1.75 7.1
MgCl2 x 6 H2O 1.375 6.763
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain