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DSMZ

DSMZ 347 . SYNTROPHOMONAS SAPOVORANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 347 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave Filter Centrifuge
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 921 ml - -
Solution B 30 ml - -
Solution C 1 ml - -
Solution D 25 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
Solution G 10 ml - -
1 Dissolve ingredients of solution A, adjust pH to 7.0, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is filter-sterilized and then equilibrated with 80% N2 and 20% CO2 gas mixture for at least 15 min. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D, E, F and G are autoclaved under 100% N2 gas. To complete the medium, appropriate amounts of the solutions B to G are added to solution A in the sequence indicated. Adjust pH of complete medium to 7.2, if necessary.

Solution A #

921
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.543 3.989
MgCl2 x 6 H2O 0.33 g 0.358 1.762
NaCl 0.40 g 0.434 7.432
NH4Cl 0.40 g 0.434 8.119
CaCl2 x 2 H2O 0.05 g 0.054 0.369
Trace element solution SL-10 1.00 ml - -
Clarified rumen fluid 50.00 ml - -
Trypticase peptone
(BD BBL) 		1.00 g 1.086 -
PIPES
(SIGMA) 		15.00 g 16.287 53.863
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.4e-4 0.002
Distilled water 870.00 ml - -

Solution B #

30
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1.5 g 50 471.752
Distilled water 30.0 ml - -

Solution C #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Seven vitamins solution 1 ml - -

Solution D #

25
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-laurate 2.78 g 111.2 500.225
Distilled water 25.00 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 1.84 g 184 1251.57
Distilled water 10.00 ml - -

Solution F #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 0.3 g 30 170.808
Distilled water 10.0 ml - -

Solution G #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.3 g 30 124.905
Distilled water 10.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Clarified rumen fluid #

1000
Solution occurs in 28 other media (first defined in Medium 1310).
1 Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 10.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Centrifuge, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
PIPES 14.896 49.263
Na-laurate 2.761 12.419
CaCl2 x 2 H2O 1.827 12.429
Na2CO3 1.49 14.054
Trypticase peptone 0.993 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain