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35. ACIDITHIOBACILLUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.1 1.869
2 3.00 g 3 22.045
3 0.10 g 0.1 0.492
4 0.14 g 0.14 0.952
5
(powdered)
10.00 g 10 311.857
6 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve all ingredients, except the sulfur and adjust the pH to 4.2, then autoclave. For sterilization place the sulfur in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium.
  2. Note: Incubate statically without shaking.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 4.2
Equipment needed: Autoclave

Associated strains

All strains for this medium: 3
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Sulfur 10 311.857
KH2PO4 3 22.045
CaCl2 x 2 H2O 0.14 0.952
NH4Cl 0.1 1.869
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature mesophilic 3 strains

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