DSMZ 356 . THIOSPHAERA PANTOTROPHA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 7.9 g 7.9 55.65
KH2PO4 1.5 g 1.5 11.023
NH4Cl 0.3 g 0.3 5.609
MgSO4 x 7 H2O 0.1 g 0.1 0.406
Trace element solution SL-10 1.0 ml - -
Yeast extract 1.0 g 1 -
Agar 20.0 g 20 59.465
Distilled water 1000.0 ml - -
1 Adjust pH to 7.5 - 8.0.
2 Autoclave the yeast extract separately.
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 7.5 - 8.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 59.465
Na2HPO4 x 2 H2O 7.9 55.65
KH2PO4 1.5 11.023
Yeast extract 1 -
NH4Cl 0.3 5.609
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