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356. THIOSPHAERA PANTOTROPHA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 7.9 g 7.892 44.34
2 1.5 g 1.499 11.012
3 0.3 g 0.3 5.603
4 0.1 g 0.1 0.405
5 Trace element solution SL-10 1.0 ml - -
6 1.0 g 0.999 -
7 20.0 g 19.98 -
8 1000.0 ml 997.21 55353.5
Instructions:
  1. Adjust pH to 7.5 - 8.0.
  2. Autoclave the yeast extract separately.
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Medium type: Complex medium
Final pH: 7.8
Equipment needed: Autoclave

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.3
Agar 19.98 -
Na2HPO4 x 2 H2O 7.892 44.34
KH2PO4 1.499 11.012
Yeast extract 0.999 -
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