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DSMZ

DSMZ 358 . ACIDIANUS MEDIUM (AEROBIC)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 358 #

ml
Equipment needed: Water bath Gassing station Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.3 9.838
KH2PO4 0.28 g 0.28 2.058
MgSO4 x 7 H2O 0.25 g 0.25 1.014
CaCl2 x 2 H2O 0.07 g 0.07 0.476
FeCl3 x 6 H2O 0.02 g 0.02 0.074
Allen's trace element solution 10.00 ml - -
Yeast extract
(OXOID) 		1.00 g 1 -
Sulfur
(powder) 		5.00 g 5 155.909
Distilled water 1000.00 ml - -
1 Dissolve ingredients, except sulfur, bring medium to the boil, then cool to room temperature and adjust pH to 2.5 using 10 N H2SO4. Dispense medium under air atmosphere enriched with 1 - 10% CO2 in culture vessels suitable for anaerobic cultivation (e.g., 20 ml medium in 100 ml serum bottles) containing already the appropriate amount of sulfur. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days.
2 Note: Inoculate with 5% (w/v) culture. Incubate without shaking.

Allen's trace element solution #

1000
Solution occurs in 14 other media (first defined in Medium 88).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 180 mg 0.18 0.91
Na2B4O7 x 10 H2O 450 mg 0.45 -
ZnSO4 x 7 H2O 22 mg 0.022 0.077
CuCl2 x 2 H2O 5 mg 0.005 0.029
Na2MoO4 x 2 H2O 3 mg 0.003 0.012
VOSO4 x 2 H2O 3 mg 0.003 0.015
CoSO4 x 7 H2O 1 mg 1.0e-3 0.006
Distilled water 1000 ml - -
1 Adjust pH of final solution to 2 with 1 N HCl.

Metadata

Last modified: 13.04.23
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 2.5
Equipment needed: Gassing station, Serum bottle, Water bath
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Gas composition

  • 72 % N2
  • 18 % O2
  • 10 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 5 155.909
(NH4)2SO4 1.3 9.838
Yeast extract 1 -
KH2PO4 0.28 2.058
MgSO4 x 7 H2O 0.25 1.014
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain