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DSMZ

DSMZ 35a . THIOMONAS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 35a #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Soution A 900 ml - -
Solution B 100 ml - -
Solution C 20 ml - -
Solution D 10 ml - -
1 Solutions A, B and D are sterilized separately by autoclaving. Solution C is sterilized by filtration. After autoclaving the solutions A and B are combined and then supplemented with the sterile solutions C and D. Best growth is observed in the syneresis water of slant agar tubes.

Soution A #

900
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.1 g 0.111 2.077
KH2PO4 3.0 g 3.333 24.495
Agar , for solid medium
optional 		20.0 g 22.222 66.073
Distilled water 900.0 ml - -
1 Adjust pH to 6.0 with NaOH.

Solution B #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 0.10 g 1 4.919
CaCl2 x 2 H2O 0.14 g 1.4 9.523
Distilled water 100.00 ml - -

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 5 g 250 1007.31
Distilled water 20 ml - -

Solution D #

10
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1 g 100 -
Distilled water 10 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 3
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		19.418 57.733
Na2S2O3 x 5 H2O 4.854 19.559
KH2PO4 2.913 21.403
Yeast extract 0.971 -
CaCl2 x 2 H2O 0.136 0.925
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen obligate aerobe 1 strain
temperature mesophilic 2 strains
temperature thermophilic 1 strain