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DSMZ

DSMZ 369 . BACILLUS TUSCIAE MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 369 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 4.50 g 4.5 31.699
KH2PO4 1.50 g 1.5 11.023
NH4Cl 1.00 g 1 18.695
MnSO4 x H2O 0.01 g 0.01 0.059
MgSO4 x 7 H2O 0.20 g 0.2 0.811
CaCl2 x 2 H2O 0.01 g 0.01 0.068
Ferric ammonium citrate 5.00 mg 0.005 -
Trace element solution SL-6 3.00 ml - -
Agar , for solid medium 15.00 g 15 44.599
Distilled water 1000.00 ml - -
1 Adjust pH to 4.0. Agar if necessary 15.0 g. Distribute in 30 - 50 ml amounts in Erlenmeyer flasks and sterilize for 15 minutes at 121°C.
2 Incubate at 50°C without agitation under 5% O2, 10% CO2, 45% H2 (v/v).

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 4.0
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Gas composition

  • 45 % H2
  • 10 % CO2
  • 5 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2HPO4 x 2 H2O 4.5 31.699
KH2PO4 1.5 11.023
NH4Cl 1 18.695
MgSO4 x 7 H2O 0.2 0.811
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain