370
370 .
HELIOBACTERIUM CHLORUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 370 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 1.0 | g | 1 | 5.741 | |
| MgSO4 x 7 H2O | 1.0 | g | 1 | 4.057 | |
| Yeast extract | 10.0 | g | 10 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.0 . | |||||
| 2 Boil the medium for few minutes under nitrogen gas. Add sodium ascorbate (0.5 g) and continue to bubble with nitrogen gas. | |||||
| Sodium ascorbate | 0.5 | g | 0.5 | 2.524 | |
| 3 Adjust pH to 6.8. | |||||
| 4 Fill immediately about 10 ml medium in 15 mL hungate tubes (with rubber septum). | |||||
| 5 Autoclave at 121 °C for 15 min. | |||||
Metadata
| Last modified: | 08.05.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 6 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 10 | - |
| K2HPO4 | 1 | 5.741 |
| MgSO4 x 7 H2O | 1 | 4.057 |
| Sodium ascorbate | 0.5 | 2.524 |
| Distilled water | - | - |
