Name: NATRONOBACTERIA MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 371 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	1.00	g	1	7.348
KCl	1.00	g	1	13.414
NH₄Cl	1.00	g	1	18.695
MgSO₄ x 7 H₂O	0.24	g	0.24	0.974
CaSO₄ x 2 H₂O	0.17	g	0.17	0.987
Trace element solution SL-10	1.00	ml	-	-
Agar , if necessary	20.00	g	20	59.465
NaCl	200.00	g	200	3422.31
Na₂-glutamate	1.00	g	1	5.913
Yeast extract (Fisher BioReagents BP1422)	5.00	g	5	-
Casamino acids	5.00	g	5	-
Na₂CO₃	5.00	g	5	47.175
Distilled water	1000.00	ml	-	-
1 Add distilled water to give a final volume of 1000 ml.
2 Adjust pH to 6.5 before autoclaving!
3 Sterilize Na₂CO₃ separate from medium, add after cooling. Check final pH to be 9.0 - 9.5.
4 If agar medium is prepared, heat and dissolve agar before adding sodium chloride.

Trace element solution SL-10 #

1000

Solution occurs in 265 other media (first defined in Medium 320).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	10.0	ml	2.5	68.568
FeCl₂ x 4 H₂O	1.5	g	1.5	7.545
ZnCl₂	70.0	mg	0.07	0.514
MnCl₂ x 4 H₂O	100.0	mg	0.1	0.505
H₃BO₃	6.0	mg	0.006	0.097
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	990.0	ml	-	-
1 First dissolve FeCl₂ in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

All strains for this medium:	20
Strains with modifications:	2

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