372 .
HALOBACTERIA MEDIUM
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* Medium for Bacillus sp. DSM 21445
With 150 g/L NaCl only
Growth conditions: 37 °C
Equipment needed:
Final pH:
7.0 - 7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract
(Fisher BioReagents BP1422) |
5.00 | g | ||
|
|
Casamino acids | 5.00 | g | ||
|
|
Na glutamate | 1.00 | g | ||
|
|
KCl | 2.00 | g | ||
|
|
Na3-citrate | 3.00 | g | ||
|
|
MgSO4 x 7 H2O | 20.00 | g | ||
|
|
NaCl | 150.00 | g/L | ||
|
|
FeCl2 x 4 H2O | 36.00 | mg | ||
|
|
MnCl2 x 4 H2O | 0.36 | mg | ||
|
|
Agar | 20.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Add distilled water to give a final volume of 1000 ml. | |||||
| 2 Adjust pH to 7.0 - 7.2 | |||||