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373. METHANOGENIUM CV MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.34 g 0.33 4.428
2 4.00 g 3.884 19.102
3 3.45 g 3.35 13.59
4 0.25 g 0.243 4.538
5 0.14 g 0.136 0.925
6 0.14 g 0.136 0.78
7 18.00 g 17.476 299.024
8 Trace element solution 10.00 ml - -
9
(0.1% w/v)
2.00 ml 0.002 0.005
10 1.00 g 0.971 11.835
11
(OXOID)
2.00 g 1.942 -
12
(BD BBL)
2.00 g 1.942 -
13
(0.1% w/v)
0.50 ml 4.9e-4 0.002
14 5.00 g 4.854 57.786
15 7.50 ml 5.752 95.722
16 Vitamin solution 10.00 ml - -
17 0.50 g 0.485 2.021
18 1000.00 ml 969.133 53795
Instructions:
  1. Dissolve ingredients (except bicarbonate, isopropyl alcohol, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and adjust pH to 7.0, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add isopropyl alcohol and cysteine from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 6.8 - 7.0, if necessary.
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 988.516 54870.9
NaCl 17.485 299.19
Isopropyl alcohol 5.752 95.722
NaHCO3 4.854 57.786
MgCl2 x 6 H2O 3.884 19.102
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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