Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 250.00 | g | 250 | 4277.89 | |
NH4Cl | 0.33 | g | 0.33 | 6.169 | |
KH2PO4 | 0.33 | g | 0.33 | 2.425 | |
KCl | 0.33 | g | 0.33 | 4.427 | |
CaCl2 x 2 H2O | 0.33 | g | 0.33 | 2.245 | |
MgCl2 x 6 H2O | 0.33 | g | 0.33 | 1.623 | |
MgSO4 x 7 H2O | 4.00 | g | 4 | 16.229 | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
Na2CO3 | 1.50 | g | 1.5 | 14.153 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Yeast extract
(OXOID) |
0.05 | g | 0.05 | - | |
Trimethylamine-HCl | 5.00 | g | 5 | - | |
Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except carbonate, trace elements, yeast extract, trimethylamine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add trace elements, yeast extract, trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.4 with an anoxic sterile solution of 5% w/v Na2CO3, if necessary. | |||||
2 Note: A white precipitate that forms after autoclaving of the mineral base will dissolve again after storage at room temperature for 2 - 3 days. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Last modified: | 28.02.22 |
Source: | DSMZ |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 7.4 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 250 | 4277.89 |
Trimethylamine-HCl | 5 | - |
MgSO4 x 7 H2O | 4 | 16.229 |
Na2CO3 | 1.5 | 14.153 |
Na2S x 9 H2O | 0.5 | 2.082 |
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