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375. METHANOHALOBIUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 250.00 g 249.501 4269.17
2 0.33 g 0.329 6.157
3 0.33 g 0.329 2.42
4 0.33 g 0.329 4.418
5 0.33 g 0.329 2.24
6 0.33 g 0.329 1.62
7 4.00 g 3.992 16.196
8
(0.1% w/v)
0.50 ml 5.0e-4 0.002
9 1.50 g 1.497 14.124
10 Trace element solution SL-10 1.00 ml - -
11
(OXOID)
0.05 g 0.05 -
12 5.00 g 4.99 52.213
13 0.50 g 0.499 2.078
14 1000.00 ml 996.215 55298.2
Instructions:
  1. Dissolve ingredients except carbonate, trace elements, yeast extract, trimethylamine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add trace elements, yeast extract, trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.4 with an anoxic sterile solution of 5% w/v Na2CO3, if necessary.
  2. Note: A white precipitate that forms after autoclaving of the mineral base will dissolve again after storage at room temperature for 2 - 3 days.
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.201 55352.9
NaCl 249.501 4269.17
Trimethylamine hydrochloride 4.99 52.213
MgSO4 x 7 H2O 3.992 16.196
Na2CO3 1.497 14.124
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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