DSMZ 377a . PALAEOCOCCUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Gassing station Water bath
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 30 513.347
MgSO4 x 7 H2O 3.50 g 3.5 14.2
MgCl2 x 6 H2O 2.75 g 2.75 13.527
KCl 0.33 g 0.33 4.427
NaBr 0.05 g 0.05 0.486
H3BO3 15.00 mg 0.015 0.243
SrCl2 x 6 H2O
(0.1% w/v)
7.00 ml 0.007 0.026
(NH4)2SO4 10.00 mg 0.01 0.076
Citric acid
(0.1% w/v)
5.00 ml 0.005 0.026
KI
(0.01% w/v)
0.50 ml 5.0e-5 1.9e-4
CaCl2 x 2 H2O 0.75 g 0.75 5.102
KH2PO4 0.50 g 0.5 3.674
NiCl2 x 6 H2O
(0.1% w/v)
2.00 ml 0.002 0.015
Modified Wolin's mineral solution 10.00 ml - -
Peptone
(BD Bacto)
5.00 g 5 -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Sulfur
(powdered)
30.00 g 30 935.454
Yeast extract
(OXOID)
1.00 g 1 -
Wolin's vitamin solution 10.00 ml - -
Na2S x 9 H2O 0.50 g 0.5 2.082
Distilled water 1000.00 ml - -
1 Dissolve ingredients except sulfur, vitamins and sulfide, adjust the pH to 6.5, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur. Sterilize medium by heating cultivation vessels in a boiling water bath for 1 - 2 hours on each of 3 successive days. Add yeast extract and vitamins (sterilized by filtration) from anoxic stock solutions prepared under 100% N2 gas. Prior to inoculation, add sulfide from a neutralized stock solution (see medium 29) prepared under 100% N2 gas atmosphere.
Solution occurs in 170 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Archaea
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Filter, Gassing station, Hungate tubes, Water bath
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 5
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 30 935.454
Peptone 5 -
MgCl2 x 6 H2O 2.75 13.527
Yeast extract 1 -
KH2PO4 0.5 3.674
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Cultivation metadata from 5 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 3 5 strains
oxygen anaerobe 4 strains
temperature hyperthermophilic 3 strains
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