377b
377b .
PYROCOCCUS ST04 MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 377b #
ml
Equipment needed: Hungate tubes
Gassing station
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 13.85 | g | 13.512 | 231.215 | |
| MgSO4 x 7 H2O | 3.50 | g | 3.415 | 13.854 | |
| MgCl2 x 6 H2O | 2.75 | g | 2.683 | 13.197 | |
| KCl | 0.33 | g | 0.322 | 4.319 | |
| NaBr | 0.05 | g | 0.049 | 0.474 | |
| H3BO3 | 15.00 | mg | 0.015 | 0.237 | |
|
SrCl2 x 6 H2O
(0.1% w/v) |
7.00 | ml | 0.007 | 0.026 | |
| (NH4)2SO4 | 10.00 | mg | 0.01 | 0.074 | |
|
Citric acid
(0.1% w/v) |
5.00 | ml | 0.005 | 0.025 | |
|
KI
(0.01% w/v) |
0.50 | ml | 4.9e-5 | 1.9e-4 | |
| CaCl2 x 2 H2O | 0.75 | g | 0.732 | 4.977 | |
| KH2PO4 | 0.50 | g | 0.488 | 3.585 | |
|
NiCl2 x 6 H2O
(0.1% w/v) |
2.00 | ml | 0.002 | 0.015 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
|
Sulfur
(powdered) |
30.00 | g | 29.268 | 912.638 | |
|
Peptone
(BD Bacto) |
5.00 | g | 4.878 | - | |
|
Yeast extract
(OXOID) |
1.00 | g | 0.976 | - | |
| NaHCO3 | 2.00 | g | 1.951 | 23.227 | |
| Na2S x 9 H2O | 0.50 | g | 0.488 | 2.031 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except sulfur, peptone, yeast extract, bicarbonate, and sulfide, adjust the pH to 6.8, and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Distribute medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials that already contain the appropriate amount of sulfur. Sterilize the medium by autoclaving at 110°C for 20 min. After sterilization add peptone, yeast extract, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust the pH of the complete medium to 6.8, if necessary. | |||||
Solution occurs in 167
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
| Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Metadata
| Last modified: | 15.12.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 29.268 | 912.638 |
| Peptone | 4.878 | - |
| MgCl2 x 6 H2O | 2.683 | 13.197 |
| NaHCO3 | 1.951 | 23.227 |
| Yeast extract | 0.976 | - |
| Show more | ||
