378 .
ARTHROBACTER MEDIUM
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Selected strain: Arthrobacter sp. DSM 3681
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 1.770 | g | ||
|
|
KH2PO4 | 0.680 | g | ||
|
|
NaCl | 0.140 | g | ||
|
|
CaCl2 | 0.132 | g | ||
|
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MgSO4 x 7 H2O | 0.200 | g | ||
|
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Mannitol | 10.000 | g | ||
|
|
Yeast extract | 0.080 | g | ||
|
|
FeSO4 x 7 H2O | 2.500 | mg | ||
|
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H3BO3 | 2.900 | mg | ||
|
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CoSO4 x 7 H2O | 1.200 | mg | ||
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CuSO4 x 5 H2O | 0.100 | mg | ||
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MnCl2 x 4 H2O | 0.090 | mg | ||
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Na2MoO4 x 2 H2O | 2.500 | mg | ||
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ZnSO4 x 7 H2O | 1.200 | mg | ||
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Distilled water | 1000.000 | ml | ||
| 1 Adjust pH to 7.0. Incubate under 5% O2 + 95% N2. | |||||