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DSMZ

DSMZ 379 . CHROMATIUM TEPIDUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 379 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 10.0 mg 0.01 0.03
MgSO4 x 7 H2O 200.0 mg 0.2 0.811
CaCl2 x 2 H2O 50.0 mg 0.05 0.34
NH4Cl 400.0 mg 0.4 7.478
NaCl 400.0 mg 0.4 6.845
Ammonium acetate OR Na pyruvate 0.5 mg 5.0e-4 0.006
KH2PO4 0.5 mg 5.0e-4 0.004
NaHCO3 2.0 g 2 23.808
Na2S x 9 H2O 1.0 g 1 4.164
Trace element solution 1.0 ml - -
Deionized water 1000.0 ml - -
1 Prepare bicarbonate and sulfide as separate solutions. Sterilize all solutions by autoclaving. After cooling mix all three solutions, adding the sulfide last. Adjust pH to 7.0 and dispense in completely filled screw-capped tubes or bottles. Incubate at 500 - 1000 Lux light intensity.

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 25.0 mg 0.025 0.193
Na2MoO4 x 2 H2O 188.0 mg 0.188 0.777
VOSO4 x 2 H2O 30.0 mg 0.03 0.151
Na2WO4 x 2 H2O 2.0 mg 0.002 0.006
Deionized water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2 23.808
Na2S x 9 H2O 1 4.164
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.2 0.811
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain