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DSMZ

DSMZ 380 . MAGNETOSPIRILLUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 380 #

ml
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.68 g 0.68 4.997
NaNO3 0.12 g 0.12 1.412
L(+)-Tartaric acid 0.37 g 0.37 2.465
Succinic acid 0.37 g 0.37 3.133
Na-acetate 0.05 g 0.05 0.61
Yeast extract 0.10 g 0.1 -
Modified Wolin's mineral solution 5.00 ml - -
Fe(III) quinate solution 0.01 M 2.00 ml - -
Agar , for semi-solid medium
(BD Bacto)
optional 		1.30 g 1.3 3.865
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Na-thioglycolate 0.05 g 0.05 0.438
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except thioglycolate and vitamins) in the order given and adjust pH to 6.75 with NaOH.
2 Preparation of liquid medium: Sparge medium with 100% N2 gas for 30 -45 min and dispense under the same gas atmosphere into anoxic Hungate-type tubes to 50% of their volume. Seal vials with screw caps and gas tight butyl rubber closures. Autoclave at 121°C for 15 min. Before inoculation add thioglycolate and vitamins from stock solutions prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to a concentration of ca. 1% (v/v) O2 in the vial (e.g., add 1 ml air to a Hungate-type tube of 16 ml total volume).
3 Preparation of semi solid medium: Supplement medium with agar, bring medium to the boil and cool under 100% N2 gas atmosphere. Dispense under same gas atmosphere aliquots of 10 ml semi-solid medium into Hungate-type tubes. Prior to inoculation add thioglycolate from a 0.5% (w/v) stock solution, freshly prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to a concentration of ca. 1% (v/v) in the vial.
4 Note: Prior to inoculation media should be slightly pink in color. Strongly reduced conditions will not support growth of microaerophilic Magnetospirillum species. Use as inoculum 10% (v/v). Incubate tubes with medium without agitation in an inclined position. During growth O2 will be consumed and the pH will increase. For cultivation of magnetic cells we recommend preparation of liquid medium, while semi-solid medium is more suitable for demonstration of microaerophilic band formation and storage.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Fe(III) quinate solution 0.01 M #

1000
Solution occurs in 2 other media (first defined in Medium 380).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 4.5 g 4.5 16.648
Quinic acid 1.9 g 1.9 9.887
Distilled water 1000.0 ml - -
1 Sterilize by filtration under 100% N2 gas atmosphere.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.7
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 99 % N2
  • 1 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 7
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		1.3 3.865
KH2PO4 0.68 4.997
Succinic acid 0.37 3.133
L(+)-Tartaric acid 0.37 2.465
NaNO3 0.12 1.412
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Cultivation metadata from 7 strains BacDive

halophily non-halophilic 1 strain
pH 6 - 8 3 strains
phyla 1 6 strains
nutrition chemoorganoheterotroph 1 strain
oxygen microaerophile 5 strains
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