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DSMZ

DSMZ 380a . TELMATOSPIRILLUM (PEAT) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 380a #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.68 g 0.675 4.957
NaNO3 0.12 g 0.119 1.401
L(+)-Tartaric acid 0.37 g 0.367 2.446
Succinic acid 0.37 g 0.367 3.108
Na-acetate 0.05 g 0.05 0.605
Modified Wolin's mineral solution 5.00 ml - -
Fe(III) quinate solution 0.01 M 2.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
D-Xylose 1.00 g 0.992 6.608
Wolin's vitamin solution (10x) 1.00 ml - -
Na-thioglycolate 0.05 g 0.05 0.435
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except xylose, vitamins and thioglycolate) and adjust pH to 5.5 - 6.0. Sparge medium with 100% N2 gas for 30 -45 min to make it anoxic and dispense under the same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume. Seal vials with gas tight butyl rubber closures. Autoclave at 121°C for 15 min. Before inoculation add xylose, vitamins and thioglycolate from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. Then add sterile air (with hypodermic syringe through the rubber closure) to 1% (v/v) O2 concentration in the gas phase.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Fe(III) quinate solution 0.01 M #

1000
Solution occurs in 2 other media (first defined in Medium 380).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 4.5 g 4.5 16.648
Quinic acid 1.9 g 1.9 9.887
Distilled water 1000.0 ml - -
1 Sterilize by filtration under 100% N2 gas atmosphere.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.5 - 6.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 99 % N2
  • 1 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
D-Xylose 0.992 6.608
KH2PO4 0.675 4.957
Succinic acid 0.367 3.108
L(+)-Tartaric acid 0.367 2.446
NaNO3 0.119 1.401
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen anaerobe 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 2 strains