DSMZ 380a . TELMATOSPIRILLUM (PEAT) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.68 g 0.68 4.997
NaNO3 0.12 g 0.12 1.412
L(+)-Tartaric acid 0.37 g 0.37 2.465
Succinic acid 0.37 g 0.37 3.133
Na-acetate 0.05 g 0.05 0.61
Modified Wolin's mineral solution 5.00 ml - -
Fe(III) quinate solution 0.01 M 2.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
D-Xylose 1.00 g 1 6.661
Wolin's vitamin solution 10.00 ml - -
Na-thioglycolate 0.05 g 0.05 0.438
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except xylose, vitamins and thioglycolate) and adjust pH to 5.5 - 6.0. Sparge medium with 100% N2 gas for 30 -45 min to make it anoxic and dispense under the same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume. Seal vials with gas tight butyl rubber closures. Autoclave at 121°C for 15 min. Before inoculation add xylose, vitamins and thioglycolate from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. Then add sterile air (with hypodermic syringe through the rubber closure) to 1% (v/v) O2 concentration in the gas phase.
Solution occurs in 170 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 2 other media (first defined in Medium 380).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 4.5 g 4.5 16.648
Quinic acid 1.9 g 1.9 9.887
Distilled water 1000.0 ml - -
1 Sterilize by filtration under 100% N2 gas atmosphere.

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.5 - 6.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 99 % N2
  • 1 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
D-Xylose 1 6.661
KH2PO4 0.68 4.997
Succinic acid 0.37 3.133
L(+)-Tartaric acid 0.37 2.465
NaNO3 0.12 1.412
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen anaerobe 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 2 strains