381
381 .
LB (Luria-Bertani) MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Escherichia coli DSM 7144
* Modification: Or any conventional minimal medium supplemented with 0.2% glucose and thiamine
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C
Main sol. 381 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 10 | g | 10 | 140.687 | |
| Yeast extract | 5 | g | 5 | - | |
| NaCl | 10 | g | 10 | 171.116 | |
| Agar | 20 | g | 20 | 59.465 | |
| Glucose* | 2 | g/l | - | - | |
| Thiamin* | 2 | g/l | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Taxonomic range: | Phages |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
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Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 20 | 59.465 |
| NaCl | 10 | 171.116 |
| Tryptone | 10 | 140.687 |
| Yeast extract | 5 | - |
| Glucose | 2 | 11.101 |
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Cultivation metadata from 719 strains
| pH | 6 - 8 | 31 strains |
| pH | 8 - 10 | 19 strains |
| pH | 4 - 6 | 12 strains |
| pH | 10 - 12 | 4 strains |
| pH | 12 - 14 | 1 strain |
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