This strain uses the standard medium without modifications.
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
10 x M9 salts (per l) | 100 | ml | ||
|
MgSO4
(1 M) |
1 | ml | ||
|
CaCl2
(0.1 M) |
1 | ml | ||
|
Thiamine-HCl x 2 H2O
(filter-sterilized, 1 M) |
1 | ml | ||
|
Glucose
(20%) |
10 | ml | ||
|
Distilled water | 900 | ml | ||
1 The above solutions should be sterilized separately by filtration (thiamine, glucose) or autoclaving. | |||||
2 Adjust pH to 7.4. |