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MINERAL MEDIUM M9 for E. coli JM strains
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Selected strain: Escherichia coli DSM 5585
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Filter
Final pH:
7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
10 x M9 salts (per l) | 100 | ml | ||
|
|
MgSO4
(1 M) |
1 | ml | ||
|
|
CaCl2
(0.1 M) |
1 | ml | ||
|
|
Thiamine-HCl x 2 H2O
(filter-sterilized, 1 M) |
1 | ml | ||
|
|
Glucose
(20%) |
10 | ml | ||
|
|
Distilled water | 900 | ml | ||
| 1 The above solutions should be sterilized separately by filtration (thiamine, glucose) or autoclaving. | |||||
| 2 Adjust pH to 7.4. | |||||