DSMZ 382 . MINERAL MEDIUM M9 for E. coli JM strains

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Selected strain: Escherichia coli DSM 6722

This strain uses the standard medium without modifications.

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
10 x M9 salts (per l) 100 ml - -
MgSO4
(1 M)
1 ml 0.12 1
CaCl2
(0.1 M)
1 ml 0.011 0.1
Thiamine-HCl x 2 H2O
(filter-sterilized, 1 M)
1 ml 0.373 1
Glucose
(20%)
10 ml 2 11.101
Proline 20 mg 0.02 0.174
Distilled water 900 ml - -
1 The above solutions should be sterilized separately by filtration (thiamine, glucose) or autoclaving.
2 Adjust pH to 7.4.
Solution occurs in 2 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 60 g 60 422.657
KH2PO4 30 g 30 220.45
NH4Cl 10 g 10 186.951
NaCl 5 g 5 85.558

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.4
Equipment needed: Filter
Edit in Medium builder

Associated strains

All strains for this medium: 12
Escherichia coli DSM 6722

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 6 42.266
KH2PO4 3 22.045
Glucose 2 11.101
NH4Cl 1 18.695
NaCl 0.5 8.556
Show more

Cultivation metadata from 12 strains BacDive

phyla 1 12 strains
temperature mesophilic 12 strains