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DSMZ

DSMZ 386 . DESULFOLUTIVIBRIO MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 386 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 942 ml - -
Solution B 30 ml - -
Solution C 10 ml - -
Solution D 1 ml - -
Solution E 10 ml - -
Solution F 13 ml - -
1 Solution A is sparged 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas mixture. Solutions C and F are autoclaved under 100% N2 gas. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of the solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 7.1 - 7.4.
2 Note: When growth has started feed culture again with same amount of pyrosulfite. After a further two days repeat feeding once more.

Solution A #

942
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.212 1.56
NH4Cl 0.25 g 0.265 4.962
NaCl 1.00 g 1.062 18.165
MgCl2 x 6 H2O 0.40 g 0.425 2.089
KCl 0.50 g 0.531 7.12
CaCl2 x 2 H2O 0.15 g 0.159 1.083
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.3e-4 0.002
Distilled water 940.00 ml - -

Solution B #

30
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1.5 g 50 471.752
Distilled water 30.0 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-acetate x 3 H2O 0.3 g 30 -
Distilled water 10.0 ml - -

Solution D #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Seven vitamins solution 1 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O5
(sodium pyrosulfite) 		0.95 g 95 499.711
Distilled water 10.00 ml - -
1 Adjust pH to 7.5-8.0 with NaOH.

Solution F #

13
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.4 g 30.769 128.108
Distilled water 13.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 19.10.22
Source: DSMZ
Synonyms: DESULFOVIBRIO SULFODISMUTANS MEDIUM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.1 - 7.4
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 1.491 14.068
NaCl 0.994 17.01
Na2S2O5 0.944 4.967
KCl 0.497 6.667
MgCl2 x 6 H2O 0.398 1.956
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains