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386. DESULFOVIBRIO SULFODISMUTANS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 940.0 ml - -
2 Solution B 1.0 ml - -
3 Solution C 1.0 ml - -
4 Solution D 10.0 ml - -
5 Solution E 30.0 ml - -
6 Solution F 1.0 ml - -
7 Solution G 13.0 ml - -
8 Solution H 10.0 ml - -
9 , might need strain adjustment 2.5 g/l - -
Instructions:
  1. Solution A is sparged 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved.
  2. Solutions B, C, D and G are prepared under 100% N2 gas and autoclaved separately. Solution E is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Solutions F and H are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of the solutions B to H are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 7.1 - 7.4.
  3. Note: When growth has started feed culture again with same amount of pyrosulfite. After a further two days repeat feeding once more.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 0.213 1.563
2 0.25 g 0.266 4.972
3 1.00 g 1.064 18.203
4 0.40 g 0.426 2.093
5 0.50 g 0.532 7.135
6 0.15 g 0.16 1.085
7
(0.1% w/v)
0.50 ml 5.3e-4 0.002
8 940.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-10 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Selenite-tungstate solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 g 30 220.459
2 10.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Seven vitamins solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 30.769 128.108
2 13.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(sodium pyrosulfite)
0.95 g 95 499.713
2 10.00 ml 998.207 55408.8
Instructions:
  1. Adjust pH to 7.5-8.0 with NaOH.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.4
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.2
Na2CO3 1.491 14.068
NaCl 0.994 17.009
Na2S2O5 0.944 4.967
KCl 0.497 6.667
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains

Similar media

503. ANAEROBIC FRESHWATER (FWM) MEDIUM 92 %
298a. LUPHET1 MEDIUM 90 %
503d. SELENIIVIBRIO MEDIUM (ARSENATE) 89 %
504. ANAEROBIC SEAWATER (SWM) MEDIUM 89 %
503b. FWM MEDIUM (MODIFIED) 87 %

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