Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 942 | ml | - | - | |
Solution B | 30 | ml | - | - | |
Solution C | 10 | ml | - | - | |
Solution D | 1 | ml | - | - | |
Solution E | 10 | ml | - | - | |
Solution F | 13 | ml | - | - | |
1 Solution A is sparged 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas mixture. Solutions C and F are autoclaved under 100% N2 gas. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of the solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 7.1 - 7.4. | |||||
2 Note: When growth has started feed culture again with same amount of pyrosulfite. After a further two days repeat feeding once more. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
KH2PO4 | 0.20 | g | 0.212 | 1.56 |
NH4Cl | 0.25 | g | 0.265 | 4.962 |
NaCl | 1.00 | g | 1.062 | 18.165 |
MgCl2 x 6 H2O | 0.40 | g | 0.425 | 2.089 |
KCl | 0.50 | g | 0.531 | 7.12 |
CaCl2 x 2 H2O | 0.15 | g | 0.159 | 1.083 |
Trace element solution SL-10 | 1.00 | ml | - | - |
Selenite-tungstate solution | 1.00 | ml | - | - |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.3e-4 | 0.002 |
Distilled water | 940.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2CO3 | 1.5 | g | 50 | 471.752 |
Distilled water | 30.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na-acetate x 3 H2O | 0.3 | g | 30 | - |
Distilled water | 10.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2S2O5
(sodium pyrosulfite) |
0.95 | g | 95 | 499.711 | |
Distilled water | 10.00 | ml | - | - | |
1 Adjust pH to 7.5-8.0 with NaOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2S x 9 H2O | 0.4 | g | 30.769 | 128.108 |
Distilled water | 13.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.5 | g | 0.5 | 12.501 |
Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Vitamin B12 | 100 | mg | 0.1 | 0.074 |
p-Aminobenzoic acid | 80 | mg | 0.08 | 0.583 |
D-(+)-biotin | 20 | mg | 0.02 | 0.082 |
Nicotinic acid | 200 | mg | 0.2 | 1.625 |
Calcium pantothenate | 100 | mg | 0.1 | 0.21 |
Pyridoxine hydrochloride | 300 | mg | 0.3 | 1.459 |
Thiamine-HCl x 2 H2O | 200 | mg | 0.2 | 0.536 |
Distilled water | 1000 | ml | - | - |
Last modified: | 19.10.22 |
Source: | DSMZ |
Synonyms: | DESULFOVIBRIO SULFODISMUTANS MEDIUM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.1 - 7.4 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na2CO3 | 1.491 | 14.068 |
NaCl | 0.994 | 17.01 |
Na2S2O5 | 0.944 | 4.967 |
KCl | 0.497 | 6.667 |
MgCl2 x 6 H2O | 0.398 | 1.956 |
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