DSMZ 388 . DICTYOGLOMUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.50 g 1.5 11.023
Na2HPO4 x 12 H2O 4.20 g 4.2 29.586
NH4Cl 0.50 g 0.5 9.348
MgCl2 x 6 H2O 0.38 g 0.38 1.869
CaCl2 x 2 H2O 0.06 g 0.06 0.408
Fe(NH4)2(SO4)2 x 6 H2O 0.04 g 0.04 -
CoCl2 x 6 H2O
(0.1% w/v)
2.90 ml 0.003 0.022
Na2MoO4 x 2 H2O
(0.1% w/v)
2.40 ml 0.002 0.01
Na2SeO3 x 5 H2O
(0.01% w/v)
1.70 ml 1.7e-4 6.5e-4
MnCl2 x 4 H2O
(0.1% w/v)
2.00 ml 0.002 0.01
ZnSO4
(0.1% w/v)
2.80 ml 0.003 0.017
Yeast extract 2.00 g 2 -
Polypeptone 2.00 g 2 -
Starch
(soluble)
5.00 g 5 -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Na2CO3 1.00 g 1 9.435
L-Cysteine HCl x H2O 1.00 g 1 5.694
Wolin's vitamin solution 10.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, cysteine and vitamins), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add and dissolve carbonate and cysteine, adjust pH to 7.2, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.2, if necessary.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Starch 5 -
Na2HPO4 x 12 H2O 4.2 29.586
Yeast extract 2 -
Polypeptone 2 -
KH2PO4 1.5 11.023
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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