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DSMZ

DSMZ 388 . DICTYOGLOMUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 388 #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.50 g 1.481 10.881
Na2HPO4 x 12 H2O 4.20 g 4.146 29.206
NH4Cl 0.50 g 0.494 9.228
MgCl2 x 6 H2O 0.38 g 0.375 1.845
CaCl2 x 2 H2O 0.06 g 0.059 0.403
Fe(NH4)2(SO4)2 x 6 H2O 0.04 g 0.039 -
CoCl2 x 6 H2O
(0.1% w/v) 		2.90 ml 0.003 0.022
Na2MoO4 x 2 H2O
(0.1% w/v) 		2.40 ml 0.002 0.01
Na2SeO3 x 5 H2O
(0.01% w/v) 		1.70 ml 1.7e-4 6.4e-4
MnCl2 x 4 H2O
(0.1% w/v) 		2.00 ml 0.002 0.01
ZnSO4
(0.1% w/v) 		2.80 ml 0.003 0.017
Yeast extract 2.00 g 1.974 -
Polypeptone 2.00 g 1.974 -
Starch
(soluble) 		5.00 g 4.936 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
Na2CO3 1.00 g 0.987 9.314
L-Cysteine HCl x H2O 1.00 g 0.987 5.621
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, cysteine and vitamins), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add and dissolve carbonate and cysteine, adjust pH to 7.2, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.2, if necessary.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Starch 4.936 -
Na2HPO4 x 12 H2O 4.146 29.206
Yeast extract 1.974 -
Polypeptone 1.974 -
KH2PO4 1.481 10.881
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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