38 .
ACETOANAEROBIUM MEDIUM
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Equipment needed:
Autoclave
Gassing station
Final gas composition:
100 % N2
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Final pH:
7.0
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Tryptone | 20.00 | g | ||
|
|
Yeast extract | 10.00 | g | ||
|
|
K2HPO4 | 1.04 | g | ||
|
|
KH2PO4 | 0.68 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | ||
|
|
Na2S x 9 H2O | 0.15 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. | |||||