This medium was extracted automatically and has not been curated by experts, yet.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Tryptone | 25.0 | g | 23.365 | 328.707 | |
Yeast extract | 7.5 | g | 7.009 | - | |
Tris-HCl
(pH 7.5, 1M) |
20.0 | ml | - | 20 | |
Distilled water | 1000.0 | ml | - | - | |
1 Autoclave, cool, and then add: | |||||
MgCl2
(1M) |
5.0 | ml | 0.476 | 5 | |
Diaminopimelic acid
(1%) |
10.0 | ml | 0.1 | 0.526 | |
Thymidine
(0.4%) |
10.0 | ml | 0.04 | 0.165 | |
Glucose
(20%) |
25.0 | ml | 5 | 27.753 | |
2 Magnesium chloride should be sterilized separately by autoclaving, and the other ingredients should be sterilized separately by filtration. |
Last modified: | 15.11.21 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 5 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Tryptone | 23.365 | 328.707 |
Yeast extract | 7.009 | - |
Glucose | 5 | 27.753 |
MgCl2 | 0.476 | 5 |
Diaminopimelic acid | 0.1 | 0.526 |
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