394
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CHI 1776 MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Escherichia coli DSM 8587
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 394 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 25.0 | g | 23.365 | 328.707 | |
| Yeast extract | 7.5 | g | 7.009 | - | |
|
Tris-HCl
(pH 7.5, 1M) |
20.0 | ml | - | 20 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Autoclave, cool, and then add: | |||||
|
MgCl2
(1M) |
5.0 | ml | 0.476 | 5 | |
|
Diaminopimelic acid
(1%) |
10.0 | ml | 0.1 | 0.526 | |
|
Thymidine
(0.4%) |
10.0 | ml | 0.04 | 0.165 | |
|
Glucose
(20%) |
25.0 | ml | 5 | 27.753 | |
| 2 Magnesium chloride should be sterilized separately by autoclaving, and the other ingredients should be sterilized separately by filtration. | |||||
Metadata
| Last modified: | 15.11.21 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 5 |
Escherichia coli DSM 8587
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Tryptone | 23.365 | 328.707 |
| Yeast extract | 7.009 | - |
| Glucose | 5 | 27.753 |
| MgCl2 | 0.476 | 5 |
| Diaminopimelic acid | 0.1 | 0.526 |
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