394 .
CHI 1776 MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Tryptone | 25.0 | g | ||
|
|
Yeast extract | 7.5 | g | ||
|
|
Tris-HCl
(pH 7.5, 1M) |
20.0 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Autoclave, cool, and then add: | |||||
|
|
MgCl2
(1M) |
5.0 | ml | ||
|
|
Diaminopimelic acid
(1%) |
10.0 | ml | ||
|
|
Thymidine
(0.4%) |
10.0 | ml | ||
|
|
Glucose
(20%) |
25.0 | ml | ||
| 2 Magnesium chloride should be sterilized separately by autoclaving, and the other ingredients should be sterilized separately by filtration. | |||||