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395b. FERVIDICOCCUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.326 6.096
2 0.33 g 0.326 2.396
3 0.33 g 0.326 4.374
4 0.44 g 0.435 2.957
5 0.70 g 0.692 3.402
6 0.50 g 0.494 8.454
7 Trace element solution SL-10 1.00 ml - -
8
(OXOID)
0.50 g 0.494 -
9
(0.1% w/v)
0.50 ml 4.9e-4 0.002
10 0.80 g 0.791 9.41
11
(BD BBL)
2.00 g 1.976 -
12 Vitamin solution 10.00 ml - -
13 0.50 g 0.494 2.057
14 1000.00 ml 986.371 54751.8
15 , might need strain adjustment 5.00 g/l - -
Instructions:
  1. Dissolve ingredients (except bicarbonate, Trypticase, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add Trypticase, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are sterilized by filtration. Adjust pH of complete medium to 6.0 - 6.1, if necessary.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.1
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.211 55353.5
Trypticase peptone 1.976 -
NaHCO3 0.791 9.41
MgCl2 x 6 H2O 0.692 3.402
NaCl 0.494 8.454
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Cultivation metadata from 2 strains BacDive

pH 4 - 6 2 strains
pH 6 - 8 2 strains
phyla 1 2 strains
nutrition chemoorganoheterotroph 1 strain
nutrition organotroph 1 strain
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