398
398 .
THERMOPLASMA VOLCANIUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 398 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 3.00 | g | 3 | 22.045 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
| (NH4)2SO4 | 0.20 | g | 0.2 | 1.514 | |
| Yeast extract | 1.00 | g | 1 | - | |
| Glucose | 5.00 | g | 5 | 27.753 | |
| Sulfur , for anaerobic media only | 4.00 | g | 4 | 124.727 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH of the mineral base to about 2 with sulfuric acid and autoclave. Add glucose and yeast extract from filter-sterilized stock solutions. | |||||
| 2 Check pH to be 2 to 3. | |||||
| 3 For anaerobic growth, the acidified mineral base containing sulfur is filled into serum bottles (20 ml per 100 ml bottle) under N2 + CO2 (80 + 20) gas mixture. Then, the bottles are sterilized by tyndallisation. Yeast extract and glucose are added from separately sterilized stock solutions. | |||||
Metadata
| Last modified: | 15.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 5 | 27.753 |
| Sulfur | 4 | 124.727 |
| KH2PO4 | 3 | 22.045 |
| MgSO4 x 7 H2O | 1 | 4.057 |
| Yeast extract | 1 | - |
| Show more | ||
