Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 3.00 | g | 3 | 22.045 | |
MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
(NH4)2SO4 | 0.20 | g | 0.2 | 1.514 | |
Yeast extract | 1.00 | g | 1 | - | |
Glucose | 5.00 | g | 5 | 27.753 | |
Sulfur , for anaerobic media only | 4.00 | g | 4 | 124.727 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH of the mineral base to about 2 with sulfuric acid and autoclave. Add glucose and yeast extract from filter-sterilized stock solutions. | |||||
2 Check pH to be 2 to 3. | |||||
3 For anaerobic growth, the acidified mineral base containing sulfur is filled into serum bottles (20 ml per 100 ml bottle) under N2 + CO2 (80 + 20) gas mixture. Then, the bottles are sterilized by tyndallisation. Yeast extract and glucose are added from separately sterilized stock solutions. |
Last modified: | 15.02.22 |
Source: | DSMZ |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 3 |
Strains with modifications: | 1 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Glucose | 5 | 27.753 |
Sulfur | 4 | 124.727 |
KH2PO4 | 3 | 22.045 |
MgSO4 x 7 H2O | 1 | 4.057 |
Yeast extract | 1 | - |
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