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405. SP2 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(Difco)
1.00 g 1 -
2
(Difco)
0.20 g 0.2 -
3 0.02 g 0.02 0.244
4
(Difco)
optional
15.00 g 15 -
5
(natural or artificial)
1000.00 ml - -
Instructions:
  1. Adjust pH to 7.2.
  2. If natural sea water is used, the pH need not be adjusted.
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 24.7 g 24.7 422.638
2 0.7 g 0.7 9.39
3 6.3 g 6.3 25.56
4 4.6 g 4.6 22.626
5 1.3 g 1.3 8.843
6 0.2 g 0.2 2.381
7 1000.0 ml 998.207 55408.8
Instructions:
  1. To prevent precipitation, CaCl2 and NaHCO3 are autoclaved each as separate stock solutions in 10 ml distilled water.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar
optional
15 -
Casitone 1 -
Yeast extract 0.2 -
Sodium acetate 0.02 0.244
Sea water - -

Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
nutrition chemoorganotroph 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 1 strain
temperature thermophilic 1 strain

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J802. MODIFIED CSY-3 AGAR 76 %
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1625. TAP WATER YEAST EXTRACT MEDIUM (TWYE) 74 %
J739. ENRICHED CYTOPHAGA AGAR 74 %

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